Study on the Mechanism of Naringin in the Treatment of Femoral Head Necrosis by Network Pharmacology

该研究以网络药理学为研究对象，探讨在股骨头坏死(ONFH)治疗中柚皮苷(NR)的潜在作用机理。方法：NR的简化分子式及其靶点预测信息是通过TCMSP和Switzerl and Target Prediction数据库获得的，然后将靶点信息通过Uniprot数据库转换成人类基因名。随后利用GeneCards、OMIM及PharmGkb数据库提取与ONFH有关的靶点，并借助R软件筛选药物与疾病之间的交集靶点(版本：4.3.1)。然后将目标靶点导入STRING数据库，获取蛋白–蛋白相互作用(PPI)信息，用Cytoscape软件(版本：3.8.0)选出核心目标超过所有条件平均值，最终通过R软件(版本：4.3.1)进行富集分析。结果：识别109个作用靶点的柚子苷、1632个与ONFH有关的基因、18个与NR-ONFH有关的靶点和7个核心靶点。GO功能富集分析得到了1107个结果(P Objective: This study aims to explore the potential mechanism of naringin (NR) in the treatment of femoral head necrosis (ONFH) through online pharmacology. Methods: The simplified molecular formula and target prediction information of NR were obtained through TCMSP and Swiss Target Prediction databases, and the target information was converted into human gene names using Uniprot database. Next, targets related to ONFH were extracted using GeneCards, OMIM and PharmGkb databases, and the intersection targets of drugs and diseases were identified with the help of R software (version: 4.3.1). The intersection targets were input into the STRING database to obtain protein-protein interaction (PPI) information, and the core targets greater than the average of all conditions were screened using Cytoscape software (version: 3.8.0). Finally, enrichment analysis was conducted using R software (version: 4.3.1). Results: 109 targets of naringin, 1632 genes related to ONFH, 18 NR-ONFH-related targets and 7 core targets were selected. GO functional enrichment analysis obtained 1107 results (P < 0.05), and KEGG pathway enrichment analysis obtained 38 signaling pathways (P < 0.05), which involved p53, IL-17, cell apoptosis, TNF and necrotic apoptosis. Conclusion: Naringin may provide a potential new strategy for the treatment of ONFH.