Structure Elucidation for MALDI Mass Spectrometry Imaging Using Infrared Ion Spectroscopy
Infrared ion spectroscopy (IRIS) is a tandem mass spectrometry (MS) technique that generates structurally diagnostic vibrational spectra for mass-selected ions trapped in a mass spectrometer. Until now, IRIS applications for biological samples have primarily focused on solution-based analyses, such as body fluids (e.g., plasma and urine) and tissue homogenates, using electrospray ionization (ESI) coupled with liquid chromatography-mass spectrometry (LC-MS). In this study, we have combined matrix-assisted laser desorption/ionization (MALDI) with IRIS for the direct analysis of small molecules from biological tissues on a Fourier-transform ion cyclotron resonance mass spectrometer. We applied this technique alongside MALDI mass spectrometry imaging to analyse brain tissue from two knockout mouse models of l-lysine catabolism disorders: pyridoxine-dependent epilepsy (<i>ALDH7A1</i>) and glutaric aciduria type 1 (<i>GCDH</i>). The MALDI-IRIS platform, now available for users at HFML-FELIX, represents a significant advance in the direct structural characterization of metabolites in complex biological tissues and opens new possibilities for structure elucidation in the field of MALDI mass spectrometry imaging.
Authors
- Martin Hrabě de Angelis (ORCID: https://orcid.org/0000-0002-7898-2353)
- Helmut Fuchs (ORCID: https://orcid.org/0000-0002-5143-2677)
- Valerie Gailus-Durner
- Clara van Karnebeek (ORCID: https://orcid.org/0000-0003-3352-5631)
- Kas J. Houthuijs (ORCID: https://orcid.org/0000-0002-8205-2896)
- Giel Berden (ORCID: https://orcid.org/0000-0003-1500-922X)
- Andrej Grgic (ORCID: https://orcid.org/0000-0002-4314-4456)
- Jonathan Martens (ORCID: https://orcid.org/0000-0001-9537-4117)
- Ron A. Wevers (ORCID: https://orcid.org/0000-0003-2278-9746)
- Stefanie Leuchtenberger (ORCID: https://orcid.org/0000-0003-2475-0810)
- Udo F. H. Engelke (ORCID: https://orcid.org/0000-0002-0980-238X)
- Michiel Vandenbosch (ORCID: https://orcid.org/0000-0002-0427-416X)
- Ron M. A. Heeren (ORCID: https://orcid.org/0000-0002-6533-7179)
- Pieter C. Kooijman (ORCID: https://orcid.org/0000-0001-8868-3616)
- Jos Oomens (ORCID: https://orcid.org/0000-0002-2717-1278)
- Rob J. Vreeken
- Lara van Tetering (ORCID: https://orcid.org/0009-0003-5070-6116)
- Dirk J. Lefeber (ORCID: https://orcid.org/0000-0001-7770-8398)
- Jelle L. Schuurman (ORCID: https://orcid.org/0009-0005-0617-9253)
- Benjamin Balluf
Institutions
- Radboud University Nijmegen (NL)
- Center for Environmental Health (US)
- University Medical Center (US)
- Radboud University Medical Center (NL)
- Helmholtz Zentrum München (DE)
- Maastricht University (NL)
- Laboratory of Molecular Genetics (PL)
- Emma Kinderziekenhuis (NL)
- Institute for Molecular Science (JP)
- Amsterdam University Medical Centers (NL)
- German Center for Diabetes Research (DE)
- Technical University of Munich (DE)
- University of Amsterdam (NL)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1021/acs.analchem.5c00948
- Citations
- 2
- Primary Topic
- Mass Spectrometry Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.51
Funders
- Universiteit van Amsterdam
- Radboud Universiteit
- Universiteit Maastricht
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Radboud Universitair Medisch Centrum