Global m6A RNA and whole 5mC DNA methylation specifically contribute to cell replicative and premature senescence induced by extrinsic oxidative stress
RNA-seq showed that most of the m6A and 5mC regulators and the majority of SASP expression were downregulated. The m6A motif was conserved in replicative and premature senescence, and its methylation was higher in replicative senescence. The most differentially 5mC methylation peak was located on chromosome 19, and its methylation was higher in premature senescence. Gene regulation by m6A in replicative senescence and 5mC in premature senescence was enriched in malignant tumors.
Authors
- Min Li (ORCID: https://orcid.org/0000-0003-1330-2326)
- Caiyun Lai
- Tingting Huang (ORCID: https://orcid.org/0000-0001-5001-0782)
- Xinyu Zhang (ORCID: https://orcid.org/0009-0006-4229-7318)
- Wenjuan Zhang (ORCID: https://orcid.org/0000-0003-2922-0640)
- Wenji Zhang (ORCID: https://orcid.org/0000-0003-1500-6627)
- Wenli Cheng (ORCID: https://orcid.org/0000-0002-3176-0227)
- Jiaqi Fu (ORCID: https://orcid.org/0009-0008-7625-2251)
- Luyi Tan
- Chenyu Zhu (ORCID: https://orcid.org/0000-0002-8516-3428)
- Zhangying Wang
Institutions
- Jinan University (CN)
- Guangdong Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- mSystems
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1128/msystems.01647-24
- Citations
- 2
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Natural Science Foundation of Guangdong Province
- Earmarked Fund for China Agriculture Research System
- Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science