Identification of Pan‐Coronavirus Neutralizing Aptamers Through CRISmers Targeting the Conserved Fusion Peptide

A series of coronavirus outbreaks emphasize the necessity to develop pan-coronavirus therapeutics. Previously, the CRISmers system is developed, a Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas)-mediated cellular screening platform for the identification of RNA aptamers. In this study, CRISmers are applied to target the fusion peptide (FP) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a conserved region across coronavirus genera, with E.coli serving as the screening host. From this approach, a lead aptamer, #FP-10 is identified, which demonstrates potent pan-coronavirus neutralization activity against multiple SARS-CoV-2 variants and alphacoronaviruses (HCoV-229E and HCoV-NL63), demonstrating its potential for development into a broad-spectrum therapeutic candidate.

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Publication Details

Journal
Advanced Healthcare Materials
Published
2025-06-16
DOI
https://doi.org/10.1002/adhm.202501869
Primary Topic
SARS-CoV-2 and COVID-19 Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Identification of Pan‐Coronavirus Neutralizing Aptamers Through CRISmers Targeting the Conserved Fusion Peptide

Canjie Chen, Jincun Zhao, Chunsheng Han, Ting Huang et al.
Advanced Healthcare Materials
SARS-CoV-2 and COVID-19 Research
article

Identification of Pan‐Coronavirus Neutralizing Aptamers Through CRISmers Targeting the Conserved Fusion Peptide

Canjie Chen, Jincun Zhao, Chunsheng Han, Ting Huang, Yongshi Shi, Jingxian Zhao, Airu Zhu, Shixian Wei, Yujiao Cai, Yu Wang, Ju Zhang, Tian Tang
article en

Abstract

A series of coronavirus outbreaks emphasize the necessity to develop pan-coronavirus therapeutics. Previously, the CRISmers system is developed, a Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas)-mediated cellular screening platform for the identification of RNA aptamers. In this study, CRISmers are applied to target the fusion peptide (FP) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a conserved region across coronavirus genera, with E.coli serving as the screening host. From this approach, a lead aptamer, #FP-10 is identified, which demonstrates potent pan-coronavirus neutralization activity against multiple SARS-CoV-2 variants and alphacoronaviruses (HCoV-229E and HCoV-NL63), demonstrating its potential for development into a broad-spectrum therapeutic candidate.

Advanced Healthcare MaterialsVol. 14(21)
Shenzhen University (CN), Chinese Academy of Sciences (CN), Fudan University (CN), ShanghaiTech University (CN), First Affiliated Hospital of Guangzhou Medical University (CN), State Key Laboratory of Respiratory Disease (CN), University of Chinese Academy of Sciences (CN), Institute of Zoology (CN), Guangzhou Medical University (CN)
National Natural Science Foundation of China, China Postdoctoral Science Foundation, Guangdong Science and Technology Department, Shenzhen Municipal Science and Technology Innovation Council, National Key Research and Development Program of China
Good health and well-being
Openalex Percentile: Top 82%
SARS-CoV-2 and COVID-19 Research
DOIOpen Access PDFOpenAlex
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