Nitrite Causes Nitrosative Stress to Iron Sulfur Clusters
Nitric oxide (NO), produced by nitrite reductases or nitric oxide synthases, performs vital roles in signaling and the immune response. Iron sulfur (FeS) clusters are known targets for NO induced degradation, serving as sensors to trigger cellular responses. However, this FeS reactivity is proposed as NO specific, with no demonstrated reactivity toward nitrite, a soluble NO storage molecule. We demonstrate that synthetic FeS clusters supported by various ligands undergo facile nitrosylation by nitrite in the presence of a reductant, evidencing the nitrite reductase reactivity for FeS clusters. Moreover, a mononitrosylated Fe<sub>4</sub>S<sub>4</sub> cluster, [tempS<sub>3</sub>Fe<sub>4</sub>S<sub>4</sub>(NO)]<sup>2-</sup>, can be readily synthesized by this approach, enabling further investigation into the FeS cluster repair and decomposition under NO induced oxidative stress.
Authors
- Vincent J. Catalano (ORCID: https://orcid.org/0000-0003-2151-2892)
- Ricardo García‐Serres (ORCID: https://orcid.org/0000-0001-5203-0568)
- Jean‐Marie Mouesca (ORCID: https://orcid.org/0000-0001-7497-1430)
- Leslie J. Murray (ORCID: https://orcid.org/0000-0002-1568-958X)
- Subhadeep Bera
- Arijeet Ghude
Institutions
- Centre National de la Recherche Scientifique (FR)
- University of Nevada, Reno (US)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- CEA Grenoble (FR)
- University of Florida (US)
- Institut des langues et cultures d'Europe, Amérique, Afrique, Asie et Australie (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1021/jacs.5c05636
- Citations
- 3
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 3.34
Funders
- LABoratoires d’EXcellence ARCANE
- Agence Nationale de la Recherche
- National Institute of General Medical Sciences
- Division of Chemistry