Impact of probiotic supplementation on growth performance, hematological parameters, and antibody titer responses to Newcastle disease in vaccinated broiler chickens

The main goal of this research was to measure probiotic effects on Newcastle disease immune response and five additional performance variables. A total of 180 day-old chickens were randomly distributed into nine separate groups with twenty birds each. Six groups (G1-G6) received probiotic supplementation, while G7 remained only vaccinated, and G8 and G9 had positive (PC) and negative control groups (NC). The Newcastle disease (ND) vaccine as part of the vaccination process administered to groups G4-G7. In this research, two probiotic strains isolated at the GCU laboratory included GCU-DAB-S-41( Enterococcus faecium OR563785.1 Pro-1) alongside GCU-DAB-S-6 ( Weisella confusa OR563786.1 Pro-2). The entire research period lasted 42 days. The research found both improved feed conversion ratio (FCR) and body weight gain measurements at a significant statistical level (p<0.05). The comparison showed serum triglycerides, together with cholesterol, dropped substantially (p<0.05) when compared to the NC groups. Probiotics-supplemented groups showed substantially elevated lymphocyte levels (p< 0.05). Probiotic-fed vaccinated chickens produced higher antibody responses against Newcastle disease than control groups, according to the results of the Hemagglutination Inhibition (HI) test at p < 0.05. Research showed that intestinal villi height increased statistically (p<0.05), and VH/CD crypt ratios became elevated in all probiotic-supplemented groups.