Molecular Networking-Driven Discovery of Antifungal Azaphilone Dimers from the Marine-Derived Fungus <i>Humicola</i> sp. HK1-8
A novel azaphilone dimer, humilone A (<b>1</b>), along with its related monomer, humilone B (<b>2</b>), was isolated from cultures of the marine-derived fungus <i>Humicola</i> sp. HK1-8 using a molecular networking-guided discovery approach. Further investigation of the molecular family of azaphilone dimers led to the putative identification of four analogues, humilones C-F (<b>3</b>-<b>6</b>), based on systematic analysis of their characteristic MS/MS fragmentation patterns. Detailed fragmentation studies of the dimers revealed that the predominant cleavage fragments originated from C-C bond scission at the dimeric methylene bridge. Compound <b>1</b> displayed antifungal activity against <i>Rhizoctonia solani</i>.
Authors
- Li Shen (ORCID: https://orcid.org/0000-0003-1947-5236)
- Xia-Hao Zhu
- Zhonghui Li (ORCID: https://orcid.org/0000-0002-7835-498X)
- Chang‐Yun Wang (ORCID: https://orcid.org/0000-0002-0236-1606)
- Likui Zhang (ORCID: https://orcid.org/0000-0002-4662-1107)
- Long Chen (ORCID: https://orcid.org/0000-0001-5908-266X)
- Min Chen (ORCID: https://orcid.org/0000-0001-6702-3993)
- Sheng Wang (ORCID: https://orcid.org/0000-0003-2696-2171)
- Tong Wu (ORCID: https://orcid.org/0000-0002-4005-3479)
Institutions
- Ministry of Education of the People's Republic of China (CN)
- Wuhan Engineering Science & Technology Institute (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Ocean University of China (CN)
- Yangzhou University (CN)
- College of Marin (US)
Publication Details
- Journal
- Journal of Natural Products
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1021/acs.jnatprod.5c00531
- Citations
- 3
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 4.18
Funders
- National Key Research and Development Program of China
- National Natural Science Foundation of China - State Grid Corporation Joint Fund for Smart Grid