From inserts to chips: microfluidic culture and 3D astrocyte co-culture drive functional and transcriptomic changes in hiPSC-derived endothelial cells
Complex cell culture systems are becoming accessible; still, additional research into their properties is needed. Our data highlights the importance of the cell environment and its impact on the cellular function and gene expression profiles. Understanding these changes can improve future models and facilitate the development of more physiologically relevant platforms.
Authors
- Prateek Singh (ORCID: https://orcid.org/0000-0001-6255-8243)
- Tuuli-Maria Sonninen (ORCID: https://orcid.org/0000-0002-8833-4106)
- Hoang-Tuan Nguyen (ORCID: https://orcid.org/0000-0002-0073-5942)
- Sara Kälvälä (ORCID: https://orcid.org/0000-0001-5553-1114)
- Sanni Peltonen (ORCID: https://orcid.org/0000-0003-0874-6440)
- Šárka Lehtonen (ORCID: https://orcid.org/0000-0002-5055-9414)
- Marika Ruponen (ORCID: https://orcid.org/0000-0002-1369-9565)
Institutions
- University of Eastern Finland (FI)
- Finnair (Finland) (FI)
Publication Details
- Journal
- Fluids and Barriers of the CNS
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1186/s12987-025-00672-7
- Primary Topic
- Barrier Structure and Function Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Itä-Suomen Yliopisto
- Suomen Kulttuurirahasto
- Päivikki ja Sakari Sohlbergin Säätiö
- Business Finland