Evaluation of Anti-Inflammatory Activity of Gloriosa Superba Using Various In-Vitro Methods

Background: Inflammation is a biological response to harmful stimuli, and traditional medicinal plants have been widely explored for their anti-inflammatory potential. Gloriosa Superba, a medicinal plant, is known for its therapeutic properties. This study evaluates the in-vitro anti-inflammatory activity of Gloriosa Superba tubers. Methods: The anti-inflammatory activity of the ethanolic extract of Gloriosa Superba tubers was assessed using the human red blood cell (HRBC) membrane stabilization method, including heat-induced hemolysis and hypotonicity-induced hemolysis, along with an egg albumin denaturation assay. Acetylsalicylic acid (ASA) was used as a standard reference drug. Results: The crude ethanolic extract exhibited significant anti-inflammatory activity in a concentration-dependent manner. The heat-induced membrane stabilization method revealed that the extract (500 μg/ml) and ASA (500 μg/ml) exhibited 52.667% and 78% inhibition, respectively. The hypotonicity-induced hemolysis method showed 35.67% and 59% inhibition of RBC hemolysis, respectively. The egg albumin denaturation assay demonstrated that the crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) inhibited egg albumin denaturation by 60% and 97.12%, respectively. Conclusion: The tubers of Gloriosa Superba exhibit promising anti-inflammatory activity, supporting their potential medicinal application. Further in-vivo studies are recommended to establish its efficacy and safety.