A combination of recombinase polymerase amplification with CRISPR technology rapidly detects goose parvovirus with high accuracy and sensitivity
This study established a highly field-deployable RPA-CRISPR/Cas12a assay for rapid, visual detection of GPV with outstanding sensitivity and specificity. Its capability for instrument-free on-site diagnosis via blue light transillumination makes this approach particularly promising for resource-limited settings.
Authors
- Meiqing Huang (ORCID: https://orcid.org/0000-0002-1491-5143)
- Shaoying Chen (ORCID: https://orcid.org/0000-0002-9781-9586)
- Xiuqin Chen (ORCID: https://orcid.org/0000-0002-4027-110X)
- Shizhong Zhang (ORCID: https://orcid.org/0000-0001-8408-2960)
- Shao Wang (ORCID: https://orcid.org/0009-0007-7699-3183)
- Su Lin (ORCID: https://orcid.org/0000-0003-1150-1356)
- Shilong Chen (ORCID: https://orcid.org/0000-0002-6533-8905)
Institutions
- Fujian Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-06-16
- DOI
- https://doi.org/10.3389/fcimb.2025.1566603
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00