An Efficient Organoid Cutting Method for Long-Term Culture and High-Throughput Analyses
This novel organoid cutting and arraying method overcomes limitations in long-term organoid culture and high-throughput processing. The simplicity of the cutter design and handling make it a versatile tool for diverse types of organoids. By enhancing organoid viability and enabling consistent sample preparation, this approach facilitates improved organ development and disease modeling, drug screening, and high-throughput analyses, including single-cell spatial transcriptomics applications.
Authors
- Marina V. Pryzhkova (ORCID: https://orcid.org/0000-0002-3462-5768)
- Kristin H. Gilchrist (ORCID: https://orcid.org/0000-0003-3584-9832)
- Nicholas Chartrain (ORCID: https://orcid.org/0000-0002-2799-0580)
- Philip W. Jordan (ORCID: https://orcid.org/0000-0003-4890-2647)
- Juliana I. Candelaria (ORCID: https://orcid.org/0000-0002-3164-5421)
Institutions
- Henry M. Jackson Foundation (US)
- Johns Hopkins University (US)
- Uniformed Services University of the Health Sciences (US)
- The Geneva Foundation (US)
- The University of Adelaide (AU)
Publication Details
- Journal
- Tissue Engineering and Regenerative Medicine
- Published
- 2025-06-16
- DOI
- https://doi.org/10.1007/s13770-025-00731-y
- Citations
- 1
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.67
Funders
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development