In vitro micropropagation of insulin (Costus pictus)

The present investigation has been conducted on “In vitro micropropagation of Insulin (Costus pictus) from nodal bud was undertaken to identify suitable media combinations for micropropagation. The mother plant of Costus pictus was collected from Sai Nursery, Chinchpur. Tal: Rahata, Dist: Ahmednagar, Maharashtra. The explants were initiated on MS media with BAP (0.5 mg/l BAP, 1 mg/l BAP, 1.5 mg/l BAP, 2 mg/l BAP, 2.5 mg/l BAP) and IAA (0.5 mg/l IAA). The best results of explants initiation were obtained on MS media containing 1.5 mg/l BAP and 0.5 mg/l IAA, after 2 weeks of culture. Multiplication was observed after 6 weeks of growth cycle. The best result of multiplication was observed for 1.5 mg/l BAP AND 1.5 mg/l IBA hormonal combination. Best result of rooting was observed for MS media supplemented with 1.5 mg/l IBA and 1 mg/l IAA. After one week the culture were transferred to hardening in control condition at polyhouse.