Construction of Exogenous Green Fluorescent Protein (GFP) Gene Expression Vector and Verification of Its Expression in Tobacco Leaves

This study aims to construct an exogenous green fluorescent protein (GFP) gene expression vector and verify its expression in tobacco leaves. First, the GFP gene was inserted into a plant expression vector containing a strong promoter to drive the expression of GFP by molecular cloning technology. Subsequently, the constructed vector was transformed into Escherichia coli for amplification, and the correctness of the inserted gene was verified by restriction endonuclease analysis and sequencing. Next, the recombinant vector was transferred into tobacco cells and infected by Agrobacterium-mediated transformation. The GFP expression in tobacco leaves was observed by fluorescence microscopy. The results showed that the transgenic tobacco plants emitted strong green fluorescence under ultraviolet light, proving that the GFP gene was successfully expressed. This study provides effective tools and methods for plant genetic engineering and lays a foundation for further research on plant cell biology.