Extraction and fermentation of galactomannon from fenugreek for the production of bio active secondary metabolites

Galactomannon extracted from Fenugreek was characterised by FTIR and NMR. The galactomannaon incorporated fermentation medium was used for secondary metabolite production. S.lactis, Yeast and Streptomyces sp were selected as fermenting microbes. The compound was extracted from fermentation by solvent extraction with ethylacetate and was used for in vitro studies. The FTIR reveals representative peaks of polysaccharide in region of 3436, 2924, 2854 and 1644 cm−1. Ethanol production was fond only on yeast fermentation. Anti diabetic data reveals that the percentage of glycation inhibition by Streptomyces was 70 and by S. lactis was 45 with 76 and 26 % of amylase inhibition respectively. Streptomyces sp showed 75 % antioxidant and 66% anti-inflammatory response. The compound extracted from S. lactis also showed 64 % free radical scavenging and 51% anti-inflammatory response. The antiulcer study revealing 27, 62, 82% of ATPase inhibition was recorded respectively among yeast, Streptomyces sp and S. lactis. Further antibacterial effect toward E. coli, S. aureus and C. albicans was registered only by Streptomyces metabolite. The GC–MS profile of extract of Streptomyces fermented fenugreek medium indicated 15 separated peaks. 1,2,3-propanetricarboxylic acid, 2-Hydroxy-, triethyl ester compounds were selected as ligands for the receptor ATPas and α-amylase in the molecular docking studies. Compound propanetricarboxylic acid was identified as the potential -amylase and ATPase antagonist based on hydrogen bonding affinities with the receptor and for drug-likeness. The molecular docking analyses discussed in this study may help in the creation of powerful inhibitors that will be useful in the management of ulcer and diabetes.