Outside Back Cover: A Single‐Molecule Liposome Assay for Membrane Permeabilisation (Angew. Chem. Int. Ed. 26/2025)
A single-molecule liposome assay for membrane permeabilization using confocal microscopy paired with a fast-flow microfluidics device is reported by Scott L. Cockroft, Mathew H. Horrocks et al. in their Research Article (e202503678). Liposomes, encapsulating calcium-sensitive dye, pass through the channel aligned with the microscope. Membrane-permeabilizing agents allow calcium influx, sensitizing the dye which is detected as the liposome passes through the confocal volume. As successive liposomes pass through the volume, signals acquired indicate the permeabilising properties of the analyte.
Authors
- Krzysztof M. Bąk (ORCID: https://orcid.org/0000-0003-4813-4887)
- Mathew H. Horrocks (ORCID: https://orcid.org/0000-0001-5495-5492)
- Scott L. Cockroft (ORCID: https://orcid.org/0000-0001-9321-8997)
- Bhanu Singh (ORCID: https://orcid.org/0000-0003-3309-8772)
- Ariel Louwrier (ORCID: https://orcid.org/0000-0003-1472-5494)
- Dylan B. George (ORCID: https://orcid.org/0000-0002-1943-5309)
- Kristin Piché (ORCID: https://orcid.org/0009-0003-9409-0907)
- Daniel C. Edwards (ORCID: https://orcid.org/0000-0003-2345-4737)
- Tianxiao Zhao (ORCID: https://orcid.org/0000-0002-8078-0638)
- Ryan Ferguson (ORCID: https://orcid.org/0000-0002-9254-582X)
Institutions
- MRC Centre for Regenerative Medicine (GB)
- University of Edinburgh (GB)
Publication Details
- Journal
- Angewandte Chemie International Edition
- Published
- 2025-06-12
- DOI
- https://doi.org/10.1002/anie.202512274
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00