A Study on the Preparation and Evaluation of an Herbal Antifungal Spray

This study presents the formulation and evaluation of herbal antifungal spray developed using natural ingredients like karanja oil, neem oil, black pepper extract, camphor and other ingredient. Now a days the fungal infection is risen in humans. Along with its growth rate, the kind of fungal species also increased considerably. For this situation effective formulation is necessary to treat these fungal infections in a simple manner. The primary objective of my research project was to create a formulation for a herbal antifungal spray. Research into tropical antifungal formulations has demonstrated considerable effectiveness in the treatment of fungal infections. Compared to conventional dosage forms, these herbal drug delivery systems provide greater benefits and remarkable advantages. The spray formulation includes a combination of natural and synthetic ingredients such as Black pepper oil (Piper nigrum) exhibits potent antimicrobial properties, while neem oil (Azadirachta indica) and karanja oil (Pongamia pinnata) etc. The pH of the final product was found to be in the skin-compatible range of under 4-6 , ensuring minimal irritation and suitability for topical use. UV-Visible spectroscopy was employed to analyze the stability and phytochemical profile of the formulation, confirming the presence of active constituents from the oils, with characteristic absorbance peaks in the 250–350 nm range. Antifungal activity was evaluated using the agar well diffusion method against Candida albicans and Aspergillus niger, where the herbal spray demonstrated significant zones of inhibition, comparable to standard antifungal agents. The spray pattern was assessed for uniformity and droplet dispersion, indicating consistent and even coverage over the application area, which is critical for effective therapeutic action.