Investigation of wound healing activity of lablab purpureus L. sweet seed extract on rats

Aim: The present study was formulated to investigate the wound healing activity of lablab purpureus seed extract in rats. Objectives: The objective of the present study are :  To identify, authenticate and collection of Lablab purpureus seeds.  To extract lablab purpureus seeds using ethanol by maceration extraction.  To carry out preliminary qualitative phytochemical screening to identify phytochemical constituents.  To conduct acute oral toxicity studies of the seeds extract as per OECD 425 guidelines[1].  To evaluate the wound healing activity of Lablab purpureus beansusing the following models. Materials and Methods: The seeds of lablab purpureus were collected, dried, powdered and kept for extraction(maceration) with ethanol. Preliminary phytochemical evaluation and acute toxicity studies were carried out. The invivo wound healing was carried out by Excision, Incision and Dead space methods. The parameters like wound contraction, tensile strength and formation of collagen fibres. Result: In wound healing activity extract showed faster contraction, increase tensile strength and increased dry and wet tissue weight compared to the control group. Conclusion: Based on the result it is confirmed that the ethanolic extract of lablab purpureus shows wound healing activity in a dose-dependent manner. The high dose(400mg/kg) shows statistically significant activity