Efficient Micropropagation of Sedum sediforme and S. album for Large-Scale Propagation and Integration into Green Roof Systems

Urban expansion has led to two significant environmental challenges: the reduction in green spaces and the rise in urban temperatures, decreasing city livability. Green roofs have emerged as a sustainable solution to mitigate these issues, offering ecological and economic benefits while improving building energy efficiency. Some species of the genus <i>Sedum</i>, particularly <i>Sedum sediforme</i> and <i>Sedum album</i>, are ideal for such green infrastructure due to their non-aggressive and superficial root system, high drought tolerance, low nutrient needs, pest and disease resistance, and metabolic adaptability during dry periods. This study aims to optimize the large-scale production of two native ecotypes of <i>S. sediforme</i> and <i>S. album</i> from the Valencian Community through an efficient propagation system that enables uniform plant production in limited space. For this purpose, we have developed micropropagation systems that allow a rapid multiplication of these two species. A direct morphogenesis system was established using axenic plant shoots, and a protocol for adventitious organogenesis from leaves was also developed. These methods significantly enhance propagation speed, spatial efficiency, and plant uniformity. Notably, the metabolic plasticity of <i>S. sediforme</i> and <i>S. album</i> reduces abiotic stress during acclimatization, promoting efficient ex vitro establishment and functional integration into extensive green roof ecosystems.