Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa)

Cultivated strawberry (Fragaria × ananassa) belongs to the family Rosaceae and is an allo-octoploid species (2n = 8× = 56). Using PacBio Revio long reads of 'Seolhyang', we completed telomere-to-telomere phased genome assemblies with a size of 797 Mb with a contig N50 of 27.04 Mb. Benchmarking of the universal single-copy orthologs (BUSCO) analysis detected 99.1% conserved genes in the assembly. In addition, the average long terminal repeat assembly index (LAI) was 17.28, with high genome continuity. In this study, we identified 50 of the possible 56 telomeres across 28 chromosomes. The 'Seolhyang' genome was annotated using RNA-Seq data representing various F. × ananassa tissues from the NCBI sequence read archive, which resulted in 129,184 genes.

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Publication Details

Journal
Scientific Data
Published
2025-06-13
DOI
https://doi.org/10.1038/s41597-025-05191-6
Citations
4
Primary Topic
Berry genetics and cultivation research
Type
article
Field-Weighted Citation Impact
7.00

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article

Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa)

Hanna Park, Hyeondae Han, Seonghee Lee, Do‐Sun Kim et al.
4 citations
Scientific Data
Berry genetics and cultivation research
7.00
article

Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa)

Hanna Park, Hyeondae Han, Seonghee Lee, Do‐Sun Kim, Yoon Jeong Jang, Koeun Han, Youngjae Oh
article en
4 citations

Abstract

Cultivated strawberry (Fragaria × ananassa) belongs to the family Rosaceae and is an allo-octoploid species (2n = 8× = 56). Using PacBio Revio long reads of 'Seolhyang', we completed telomere-to-telomere phased genome assemblies with a size of 797 Mb with a contig N50 of 27.04 Mb. Benchmarking of the universal single-copy orthologs (BUSCO) analysis detected 99.1% conserved genes in the assembly. In addition, the average long terminal repeat assembly index (LAI) was 17.28, with high genome continuity. In this study, we identified 50 of the possible 56 telomeres across 28 chromosomes. The 'Seolhyang' genome was annotated using RNA-Seq data representing various F. × ananassa tissues from the NCBI sequence read archive, which resulted in 129,184 genes.

Scientific DataVol. 12(1)
Chungbuk National University (KR), Rural Development Administration (KR), University of Florida (US), Kukdong Communication (South Korea) (KR), University of West Florida (US)
National Research Foundation, Rural Development Administration, National Research Foundation of Korea, Ministry of Science and ICT, South Korea
Openalex Percentile: Top 4%
Berry genetics and cultivation research
7.00
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Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) — Hanna Park, Hyeondae Han, et al. · Scientific Data (2025) | TGRS Research Map | TGRS