Proteomic analysis of hydrogen peroxide-treated human chondrocytes shows endoplasmic reticulum stress, cytoskeleton remodeling, and altered secretome composition
Our study demonstrates a detailed proteomic view of the stress response of H<sub>2</sub>O<sub>2-</sub>treated chondrocytes, highlighting the significant changes in ER function, cytoskeletal remodeling, protein secretion, and immune responses. These changes suggest that oxidative stress impacts ECM balance and can contribute to cartilage disorders, such as OA, through different mechanisms than what is usually observed with inflammatory stimulus, offering new insights into the molecular mechanisms underlying oxidative stress in chondrocytes.
Authors
- Miryam Paola Alvarez‐Flores (ORCID: https://orcid.org/0000-0002-7986-923X)
- Giuseppe Palmisano (ORCID: https://orcid.org/0000-0003-1336-6151)
- Marcus V. Buri (ORCID: https://orcid.org/0000-0002-8925-1206)
- Marcelo Medina de Souza (ORCID: https://orcid.org/0000-0003-1706-9285)
- Deivid Martins Santos (ORCID: https://orcid.org/0000-0003-3632-1167)
- Hugo Vigerelli (ORCID: https://orcid.org/0000-0002-8197-2260)
- Hellen Paula Valerio (ORCID: https://orcid.org/0000-0002-9042-3319)
- Ana Marisa Chudzinski‐Tavassi (ORCID: https://orcid.org/0000-0001-7717-7013)
- Thaís Regina Toledo (ORCID: https://orcid.org/0000-0003-1626-8944)
- Thatiana Corrêa de Melo (ORCID: https://orcid.org/0000-0001-7002-5180)
- Amanda Teixeira de Melo (ORCID: https://orcid.org/0000-0003-3867-8522)
- Renata Nascimento Gomes (ORCID: https://orcid.org/0000-0003-0474-1896)
Institutions
- Universidade de São Paulo (BR)
- Instituto Butantan (BR)
Publication Details
- Journal
- Cell Communication and Signaling
- Published
- 2025-06-13
- DOI
- https://doi.org/10.1186/s12964-025-02291-z
- Citations
- 6
- Primary Topic
- Endoplasmic Reticulum Stress and Disease
- Type
- article
- Field-Weighted Citation Impact
- 6.91
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior