Abstract 2052 Single-molecule fluorescence reveals the activation mechanism of the human A2A adenosine receptor in native lipid environment

The human A2A adenosine receptor (A2AAR) is a G-protein coupled receptor (GPCR) involved in many physiological processes, such as cardiovascular regulation, immune modulation, and neuromodulation. It is also a therapeutic target for treating neurodegenerative diseases such as Parkinson's and certain types of cancer. The A2AAR can sense external stimuli or ligands that trigger conformational changes that activate intracellular signaling pathways. Membrane mimetics such as nanodiscs are widely used to study such receptors, as they maintain the native membrane-like environment, which influences the dynamic nature of the receptor and its interactions binding with G-protein and other affecters.