Abstract 1022 Uncovering Functions of stress induced Enzymes

Cells possess intricate metabolic networks that rely on hundreds of enzymes to function correctly.Despite extensive research, many of these enzymes remain uncharacterized, and identifying them is crucial for advancing our understanding of cellular and organismal metabolism.However, many of these enzymes are not expressed in standard conditions making it challenging to study their activity.To overcome this challenge, we constructed a collection of yeast strains each expressing an uncharacterized enzyme fused to a green fluorescent protein.Focusing on those not expressed in standard conditions we subjected them to various growth or stress environments to identify expression patterns indicative of their metabolic functions.This approach allowed us to identify the biologically relevant conditions for studying each uncharacterized protein.Under these conditions, we depleted the uncharacterized enzymes and compared the cellular response via metabolomics.By examining the metabolic changes that follow protein depletion in these conditions, we expect to understand the protein's function.Uncovering new enzyme functions has implications for multiple fields, providing tools for studying cell biology and biotechnology, and is expected to expand our understanding of metabolism in eukaryotes.