Abstract 1703 An Expression and Purification Method for Structurally Characterizing KCNE4 in vitro
Members of the KCNE family are accessory subunits that modulate voltage-gated potassium channels. KCNE4, in particular, is known to inhibit potassium ion currents in these channels. However, its structure, dynamics, and mechanism of inhibition remain poorly understood, likely due to difficulties in overexpressing and purifying the protein. In this study, we developed and validated an alternative expression and purification protocol to obtain overexpressed KCNE4 for in vitro analysis. Validation was performed using SDS-PAGE, CW-EPR, CW-EPR power saturation, and CD spectroscopy.
Authors
- Carole Dabney‐Smith (ORCID: https://orcid.org/0000-0003-1347-3870)
- Andrew Morris
- Elizabeth L. Travis (ORCID: https://orcid.org/0000-0003-1140-1822)
- A. Bates (ORCID: https://orcid.org/0009-0005-7313-0345)
- Robert M. McCarrick (ORCID: https://orcid.org/0000-0002-0392-0051)
- Gary A. Lorigan (ORCID: https://orcid.org/0000-0002-2395-3459)
- Ilsa Miller
- Indra D. Sahu (ORCID: https://orcid.org/0000-0002-7333-0356)
Institutions
- University of Minnesota System (US)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.jbc.2025.109408
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Institute of General Medical Sciences