Abstract 2261 Cytosolic NADK is conditionally essential for folate-dependent nucleotide synthesis in human cells
NADPH is a cell-essential coenzyme that provides the major source of reducing equivalents to power macromolecule biosynthesis and antioxidant defense. The rate-limiting substrate for NADPH production is its oxidized form, NADP+, which is generated from NAD+ by NAD kinases. NADP+ and NADPH (NADP(H)) each exist in mutually exclusive pools in the mitochondria and cytosol of human cells, with compartmentalized levels of NADP(H) being controlled by two distinct NAD kinases (NADK, cytosolic; NADK2, mitochondrial).
Authors
- Judith Simcox (ORCID: https://orcid.org/0000-0001-7350-6342)
- Kyle M. Flickinger (ORCID: https://orcid.org/0009-0003-3538-5887)
- Jason R. Cantor (ORCID: https://orcid.org/0000-0001-5263-2059)
- Gina Wade (ORCID: https://orcid.org/0009-0000-1773-1393)
- Kimberly S. Huggler (ORCID: https://orcid.org/0000-0003-0289-3167)
- Gavin Chang
- Carlos Mellado Fritz
Institutions
- University of Wisconsin–Madison (US)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.jbc.2025.109514
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Howard Hughes Medical Institute
- University of Wisconsin-Madison
- National Institutes of Health