Abstract 1438 Cholesterol blocks the assembly and oncogenic signaling of the EphA2 receptor

The receptor tyrosine kinase EphA2 has low expression in adult tissues, with the exception of tumors, where it promotes malignancy in different cancer types, with a salient role in metastasis. At the molecular level, EphA2 can be found in different self-assembly states: as a monomer, dimer and oligomer. However, we have a poor understanding of which specific EphA2 state is responsible for oncogenic signaling, which is characterized by phosphorylation of cytoplasmic serine residues. To address this question, we have developed SiMPull-POP, a new single-molecule method for accurate quantification of the self-assembly of membrane proteins in eukaryotic membranes.