Abstract 2491 Functional Characterization of ATP-Citrate Lyase Mutants in Yarrowia lipolytica
ATP citrate lyase catalyzes the ATP-dependent conversion of citrate to acetyl-CoA, an indispensable intermediate in lipid biosynthesis. The Yarrowia lipolytica ACL enzyme is a heterodimeric protein composed of the catalytic subunit Acl1 and the regulatory subunit Acl2. Mutants deficient in ACL exhibit diminished lipid levels and a global transcriptional reprogramming. In this work, we investigated the phenotypic effects of the ablation of each ACL subunit on growth and carbon source utilization.
Authors
- Stylianos Fakas (ORCID: https://orcid.org/0000-0001-8692-1326)
- Ayodeji Odunsi (ORCID: https://orcid.org/0000-0003-4457-7648)
Institutions
- Florida Agricultural and Mechanical University (US)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.jbc.2025.109435
- Citations
- 1
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.81