Abstract 2423 Feedback regulation of NO production on arginine metabolism in macrophages

Macrophages are essential innate immune cells that reprogram arginine metabolism to support immune responses. Upon stimulation by bacterial components (e.g., lipopolysaccharide, LPS) and cytokines (e.g., interferon-gamma, IFNγ), macrophages upregulate nitric oxide synthase 2 (Nos2) to produce inducible nitric oxide synthase (iNOS), which converts arginine into citrulline and nitric oxide (NO). NO contributes to pathogen killing and regulates key cellular processes, while metabolic intermediates and enzymes in this pathway play critical roles in immune responses.