Abstract 1725 Exploring the Role of Phosphorylation in Malate Dehydrogenase: Enzymatic Analysis of Phosphomimetic Mutant S227D

Malate dehydrogenase (MDH) is a key enzyme in the citric acid cycle and thus plays a central role in whole-body metabolic regulation. Beyond its classical role as the catalytic interconverter of substrates malate and oxaloacetate (OAA), MDH has demonstrated a high-degree of adaptability to changing cellular demands and conditions. Under hypoxic conditions, MDH has been known to participate in a promiscuous reaction with α-ketoglutarate (α-KG) to form L-2-Hydroxyglutarate (L-2-HG), an oncometabolite that promotes tumorigenesis.