Abstract 2350 Characterization of New Molecular Tools to Detect Branched Ubiquitin Chains

Ubiquitin chains are key regulatory signals that control the stability, activity, and location of proteins in eukaryotes. Recent studies have shown that ubiquitin chains can be branched and that branched chains regulate proteins through diverse mechanisms. Despite mounting evidence that branched chains have important biological functions, our understanding of these signals has been limited by a lack of accessible methods to detect them. In this study, we explored the effectiveness of two newly developed tools designed to detect branched K48/K63 chains.