Abstract 2566 Intracellular pH regulates kinase signalling

Protein kinases, assembled into phosphorylation relays, transmit and process biochemical information for cells to respond appropriately to their environment. It remains unclear how diverse responses are encoded within a limited number of kinases involved in signal transduction. Here, we investigated the influence of pH, a fundamental property in nature, on c-Jun N-terminal kinases (JNK), members of the Mitogen-Activated Protein Kinase (MAPK) family integral to cell responses to stress stimuli. Our quantitative imaging of biosensors for pH and JNK activity in live cells revealed that intracellular pH (pHi) was a significant regulator of JNK signalling.