Abstract 2595 A spastic paraplegia-related DDHD2 functions as a lipase and transacylase that can remodel the acyl-chains of triglycerides

Objective: This study aims to elucidate the substrate specificity of DDHD2 as a lipase towards phospholipids and neutral lipids, and to define its molecular mechanisms to enhance our understanding of this enzyme's function. Methods: We employed protein purification, enzyme activity assays, liposome co-sedimentation assays and liquid chromatography-mass spectrometry (LC-MS) to investigate DDHD2's substrate specificity and enzymatic mechanisms. Results: DDHD2 exhibited a marked preference for hydrolyzing diacylglycerol (DAG) over phospholipids.