Abstract 2927 Identifying Histone Citrullination Sites in vitro via NMR Spectroscopy

Citrullination is a charge-modifying post-translational modification (PTM) whereby arginine is converted to the non-coded amino acid citrulline by calcium-activated protein arginine deiminases (PADs; EC 3.5.3.15). In this enzyme class, there are five known PAD isozymes found in humans (1, 2, 3, 4, and 6) that are differentially expressed and have distinct targets including histones. While some histone citrullination sites are known, a comprehensive investigation of all histone arginine targets by each PAD is lacking.