Abstract 2872 Enhancing transfection efficiency of primary cells through lipid nanoparticle-mediated delivery

Modification of cells is critical for a diverse range of research endeavors, spanning disease modeling, therapeutic target discovery, immunotherapy, and vaccine development. Primary cells, in contrast to immortalized cell lines, more faithfully mimic in vivo targeting of nanoparticles, a pivotal aspect for advancing therapeutic strategies. However, genetic manipulation of primary cells is a challenging, costly, and time-consuming process. T cells, in particular, exhibit low transfection efficiency and poor viability when treated with common chemical transfection reagents.