Abstract 2772 Enabling Long Read Sequencing by Automating DNA Shearing and SMRTbell Library Construction on the Biomek i7 Hybrid Workstation

One means to further advance genetic discovery is through the utilization of long-read sequencing where larger DNA fragments (1k to 20K in length) can be analyzed as a single genetic component and reduce the complexity of genetic assembly of sequencing data. The PacBio SMRTbell® prep kit 3.0 for whole genome and metagenome libraries is a 5-step process that enables sequencing on PacBio® long-read systems Sequel II/IIe or Revio. A variety of sample inputs including human, plant, animal, microbial, or metagenomic can be used.

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Publication Details

Journal
Journal of Biological Chemistry
Published
2025-05-01
DOI
https://doi.org/10.1016/j.jbc.2025.109783
Primary Topic
Cancer Genomics and Diagnostics
Type
article
Field-Weighted Citation Impact
0.00
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article

Abstract 2772 Enabling Long Read Sequencing by Automating DNA Shearing and SMRTbell Library Construction on the Biomek i7 Hybrid Workstation

P. S. Banerjee, Martin Giner, Christopher P. Conner, Gregory Young et al.
Journal of Biological Chemistry
Cancer Genomics and Diagnostics
article

Abstract 2772 Enabling Long Read Sequencing by Automating DNA Shearing and SMRTbell Library Construction on the Biomek i7 Hybrid Workstation

P. S. Banerjee, Martin Giner, Christopher P. Conner, Gregory Young, Ferris Jung, Kelly Parliament
article en

Abstract

One means to further advance genetic discovery is through the utilization of long-read sequencing where larger DNA fragments (1k to 20K in length) can be analyzed as a single genetic component and reduce the complexity of genetic assembly of sequencing data. The PacBio SMRTbell® prep kit 3.0 for whole genome and metagenome libraries is a 5-step process that enables sequencing on PacBio® long-read systems Sequel II/IIe or Revio. A variety of sample inputs including human, plant, animal, microbial, or metagenomic can be used.

Journal of Biological ChemistryVol. 301(5)
Meiji Pharmaceutical University (JP)
Openalex Percentile: Top 82%
Cancer Genomics and Diagnostics
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