Abstract 1374 Purifying a DAG Biosensor to Quantify PLC Activity at Membranes

Studying phospholipase C (PLC) enzymes is important to help us understand cardiovascular disease, the leading cause of death worldwide. PLC interacts with the inner leaflet of the plasma membranes in cells, where it hydrolyzes the lipid phosphatidylinositol bisphosphate (PIP2) into two molecules: diacylglycerol (DAG) and inositol trisphosphate (IP3). IP3 initiates the release of intracellular calcium, while DAG activates another enzyme, protein kinase C (PKC). The dysregulation of intracellular calcium and PKC is associated with cardiovascular disease.