Abstract 2833 Expanding the β-Arrestin's Interactome: Convolutional Prediction and BRET Validation of the “arreSTick” Motif

The binding and function of β-arrestins in G protein-coupled receptors (GPCRs) are governed by specific phosphorylation motifs, although the precise arrangement of phosphorylated residues underlying a stable association remains elusive. We used a one-dimensional (1D) sequence convolution model, trained on GPCRs with characterized β-arrestin-binding properties, to identify a distinct amino acid pattern - termed “arreSTick” - that mediates stable interactions with β-arrestins. Remarkably, we also detect this arreSTick motif in numerous non-receptor proteins.