Abstract 1113 Exploring the Potential of Hybridization Chain Reaction (HCR) and Catalytic Hairpin Assembly (CHA) Technologies in Clinical Dengue Diagnostics

Dengue fever is one of the most important vector-borne diseases in Taiwan, and it has caused more than 26,000 cases in 2023. Although quantitative reverse-transcription polymerase chain reaction (qRT-PCR) provides a sensitive molecular diagnosis of dengue, the reaction requiring precise and expensive equipment and reagents has limited its use in the field. Hybridization chain reaction (HCR) represents one of the simplest forms of non-enzymatic signal amplification strategies that employs toehold-mediated nucleic acid strand displacement for the recognition of specific targets and repetitive stacking of hybridized strands for the accumulation of signals.