Abstract 2689 Visualizing PINK1 Activity Dynamics with a Phase Separation-Based Kinase Activity Reporter

Behind mitochondrial function is phosphatase and tensin homologue-induced kinase 1 (PINK1), a serine/threonine kinase that plays roles in mitophagy, cell death, and regulation of cellular bioenergetics. Current approaches to studying PINK1 activity depend on bulk approaches that provide a snapshot of activity, and can miss the dynamics and heterogeneity of PINK1 activity. Therefore, we sought to temporally characterize PINK1 activity by developing a novel PINK1 kinase activity reporter (KAR). In particular, KARs have been developed to characterize the activity of kinases such as protein kinase A, ERK, Akt, and many others at the single cell level.