Abstract 2166 Development of a Simple Protocol for Accurate Quantification of GFP Protein Using a Microplate Reader

GFP (green fluorescent protein) is a popular reporter for visualization and measurement of relative gene expression in cells or animals in situ. However, currently GFP measurement is relative and accurate quantitative measurement of GFP protein levels in cells and tissues remains a challenge, especially for inter-study comparisons of GFP expression levels. We aimed to establish a fast and reliable protocol for accurate quantification of GFP protein in animal tissues using a standard fluorescence microplate reader.