Abstract 2112 Transient miRISC-mRNA interactions within higher order RNP assemblies promote translational repression

aptamer.The oligonucleotide pool is progressively enriched by adjusting the ratio to include more N71 RNA pool and less SOD1 target, ensuring that only the highest-affinity aptamers bind effectively.To further enhance stringency, additional washes with higher wash volumes have been implemented, increasing the selectivity for aptamer-target binding.After 5-6 rounds of SELEX, the enriched RNA pool will be cloned and sequenced after the fifth or sixth round of selection to assess whether a viable aptamer targeting SOD1 has been identified.Once a successful aptamer for SOD1 is identified, the project will move forward to develop an aptamer targeting the biomarker pirenzepine.These two aptamers will then be utilized through aptamer-based targeted delivery within a therapeutic to alleviate symptoms of multiple sclerosis.Thank you for the generous support of the UT Freshman Research Initiative in providing the laboratory resources and materials essential for conducting this project.