Abstract 2906 Evaluation of Osteogenic Molecules in Mesenchymal Stem Cells Differentiated into Osteoblasts Cultured on Silica and 3D Printed PLA Scaffolds for Bone Graft Formation

Objective: This study aims to evaluate the expression of key osteogenic molecules SPARC, BMP2, and RUNX2 in mesenchymal stem cells (MSCs) differentiated into osteoblasts and cultured on silica-based and 3D-printed PLA scaffolds to determine their potential for bone graft applications. Methods: MSCs were expanded and induced to differentiate into osteoblasts using osteogenic medium. Cells were seeded onto silica and PLA scaffolds fabricated via 3D printing. Osteogenic differentiation was assessed by quantifying the gene expression levels of SPARC, BMP2, and RUNX2 using qRT-PCR.