Abstract 1139 Shedding light on the dark kinome: Elucidating the regulation and physiological function of an odd tyrosine kinase

The complexity and specificity of cellular processes, such as signal transduction and metabolism, require spatial microcompartmentation and dynamic modulation of the underlying biochemical activities.We hypothesize that cellular biochemical activities are spatially organized into an "activity architecture" and reorganization and restructuring of this activity architecture lead to disease.In this talk, I will introduce a series of genetically encoded or chemigenetic fluorescent biosensors that we have developed to monitor biochemical events in living cells, and then present a couple of studies where we combine quantitative fluorescence imaging with targeted perturbations as well as biochemical and functional assays to probe the spatiotemporal regulation of cell signaling and metabolism.