Abstract 1236 Development and Initial Characterization of Next Generation Genetically Encodable Fluorescent Biosensors of RSK, S6K, and ROCK Activity

Human diseases such as cancer, diabetes, and various neurological disorders can result from the dysregulation of numerous interrelated signaling pathways. To enhance our capacity to predict, diagnose, and treat these diseases, it is essential to understand the complex interactions among various signaling components under diverse biological settings. Molecular imaging techniques, such as genetically encodable fluorescence resonance energy transfer (FRET)-based biosensors, have proven pivotal for the real-time visualization of dynamic signaling processes in living cells and organoids.