Abstract 2067 Actin-Regulatory Protein Profilin1 Plays an Important Role in Lipid Homeostasis

Objective: Phospholipid dysregulation is a major contributor to promoting oncogenesis, malignant behavior, therapeutic resistance, and metabolic plasticity in cancer. The present work explores the unconventional role of profilin1 (Pfn1), an actin monomer-binding protein whose expression is altered in various human cancers and is traditionally recognized for its role in cellular actin dynamics, in lipid regulation in cancer cells. Methods: We used both gain- and loss-of-function (overexpression, RNAi, inducible CRISPR/Cas9-mediated gene deletion) strategies in our studies.