Abstract 1564 Determining the role of Trm7, Trm732, and Trm734 in tRNA binding

Modifications of the tRNA anticodon loop are important to translation. Proteins Trm7, Trm732, and Trm734 work together to modify the anticodon loop of tRNAPhe. Trm7 plays a catalytic role in methylation activity, and Trm732 and Trm734 are predicted to bind and position tRNAPhe for methylation at nucleotides C32 and G34 in Saccharomyces cerevisiae. Lack of Trm7 causes a sick phenotype in S.cerevisiae, and mutations in the TRM7 human ortholog, FTSJI, cause non-syndromic X-linked intellectual disability.