Abstract 2918 Detection challenges in distinguishing genetically engineered and non-genetically engineered food products

Genetically modified organisms (GMOs) are controversial globally due to health concerns and the bioengineering involved in developing certain seed products. This study aimed to validate manufacturer claims regarding genetic engineering in various food products labeled as either genetically engineered or non-GMO. DNA was extracted from corn syrup, corn off the cob, natural corn seed, and grits using both a Qiagen kit and an in-house method. Polymerase chain reaction (PCR) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) were employed to verify the presence of GMO proteins, specifically targeting the CaMV35S promoter, Bt-176 gene, MON18 gene, and Nos terminator.