USE OF MULTILOCAL MARKERS TO CONFIRM THE UNIQUENESS OF LEBEDYN CATTLE

The aim of this study is to analyze the informativeness of ISSR markers in the study of intrapopulation genetic polymorphism of the Lebedyn local breed cattle population. Materials and methods of research. To confirm the uniqueness and consolidation of the Lebedyn breed cow population, an assessment of the genetic polymorphism of the studied cows was carried out using molecular diagnostics (ISSR-fingerprinting). The material for assessing intrapopulation genetic variability was blood samples taken from cows of the local Lebedyn breed (n = 33 heads), which are kept in the Boryspil district of the Kyiv region in the Holosiyivske farm, Ukraine. Molecular genetic studies were carried out on the basis of the аnimal genetics laboratory of the M.V. Zubets Institute of Animal Breeding and Genetics of the National Academy of Sciences of the Ukraine. Results. The genetic structure of the population of the local small-sized Lebedyn cattle breed of Ukraine was analyzed using 8 ISSR systems based on the following microsatellites: (ACC)6G, (CTC)6C, (GAG)6C, (GA)6CC, (AG)8CG, (AG)8CA, (GA)9C and (AG)9C. The spectra of amplification products using different microsatellite loci as primers annealing sites and amplification of sites located between their inverted repeats significantly differed from each other. The difference was both in the number of amplicons obtained, their length (in nucleotide pairs), and their polymorphism. 88 amplified DNA fragments were identified, of which only 18 were polymorphic. The total number of polymorphic loci was 20.45%. According to the results of population genetic analysis, the (AG)8CGmarker was the least polymorphic (PIC = 0.115). Conclusion. These results indicate a high degree of genetic consolidation and possible reproductive isolation of the population of the studied animals.