POTENTIAL ANTI-AGING AND PHENOLIC COMPOUNDS in DIFFERENT PARTS OF PLEUROTUS PULMONARIUS MUSHROOM EXTRACTS CULTIVATED ON DIFFERENT SUBSTRATES

Pleurotus mushrooms (Pleurotus spp.) are edible fungi known for their rich bioactive compounds and their ability to grow on various lignocellulosic substrates including agriculture byproducts. The unique attributes of Pleurotus spp. have gained popularity, thereby increasing the cultivation of Pleurotus mushroom among farmers. In this study, Pleurotus pulmonarius was cultivated using two different substrates sawdust substrate ((SS) and pineapple-waste substrate (PS)) and separated into caps, stems and stem waste after harvest. The mushrooms parts were extracted using 80% ethanol prior to analysis of the bioactivities and specific phenolic acids. Anti-aging activities of P. pulmonarius samples were evaluated using enzyme inhibition reactions against tyrosinase, elastase and collagenase. Quantification of phenolic acids was carried out using high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). PS-cap samples demonstrated the highest potency against tyrosinase (IC50: 21.22±0.20 mg/mL) and collagenase (IC50: 26.12 ± 1.17 mg/mL), while SS-cap exhibited the highest elastase inhibition atIC50: 36.39 ± 1.93 mg/mL. Additionally, p-coumaric acid was higher in the stem waste parts with 7.37±0.88 mg/g in SS-stem waste and 5.99±0.78 mg/g in PS-stem waste. This study presents the first report on the anti-elastase and anti-collagenase activities of Pleurotus mushroom extract and the potential bioactivities and phenolics in the stem waste extracts. In conclusion, this study highlights the bioactivities and phenolic acid compounds of Pleurotus mushroom extracts cultivated on different substrates, indicating their promising applications in various industries.