Abstract 1253 Discovering the Function/Dysfunction Switch of MitoNEET

MitoNEET is a [2Fe-2S] redox active mitochondrial protein belonging to the CDGSH iron-sulfur domain (CISD) family of proteins. MitoNEET has been implicated as potential targets for drug development to treat various disorders, including type-2 diabetes, cancer, and Parkinson's Disease. However, the specific cellular function(s) for mitoNEET still remains to be fully elucidated, and family presents a significant roadblock in rational drug development. Here we show that mitoNEET binds the enzymatic cofactor pyridoxal phosphate (PLP) specifically at only one of its eleven lysine residues, Lys55.