Abstract 1120 Substrate Channeling in Proline Metabolism

The reduction potential of flavin-bound to Sinorhibozium maloti proline utilization A (SmPutA) can provide unique and insightful information on enzyme activity and the active site environment. Flavin is crucial in biological metabolism such as aerobic respiration and other important processes like oxygen activation that are catalyzed by flavin-dependent enzymes. In this study, we seek to determine whether the redox potential of SmPutA-bound flavin can be used to characterize potential long-distance communication within SmPutA, a large bifunctional enzyme that catalyzes the overall oxidation of proline to glutamate via two catalytic domains.